产品货号:
Z15686
中文名称:
Collagen Type VI抗体
英文名称:
Anti-Collagen Type VI Antibody
产品规格:
100μg
发货周期:
1~3天
产品价格:
询价
抗体名称 | Anti-Collagen Type VI Antibody |
指标别名 | COL6A1;COL6A2;COL6A3;Alpha 1 (VI) chain (61 AA);CO6A1;COL6A1;COL6A2;COL6A3;Collagen alpha 2(VI) chain;Collagen alpha 3(VI) chain;Collagen alpha-1(VI) chain;Collagen type VI alpha 1;Collagen type VI alpha 2;Collagen type VI alpha 3;Collagen VI alpha 1 polypeptide;Collagen VI alpha 2 polypeptide;Collagen VI alpha 3 polypeptide;CollagenVI;Human mRNA for collagen VI alpha 2 C terminal globular domain;OPLL;PP3610 |
克隆性 | Polyclonal |
检验物种 | bovine,human |
应用范围 | Dot blot,IHC |
基因名称 | COL6A1/COL6A2/COL6A3 |
抗体来源 | Rabbit |
抗体类型 | IgG |
免疫原 | Collagen Type VI from human and bovine placenta |
成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2,0.01% (w/v) Sodium Azide |
纯化方式 | Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens,human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed,paraffin embedded tissues.?This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I,II,III,IV or V collagens.Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. |
浓度 | 1.0mg/mL by UV absorbance at 280nm |
产品形态 | 溶液 |
保存条件 | Store vial at 4℃ prior to opening.This product is stable at 4℃ as an undiluted liquid.Dilute only prior to immediate use.For extended storage,mix with an equal volume of glycerol,aliquot contents and freeze at -20℃ or below.Avoid cycles of freezing and thawing.Expiration date is one (1) year from date of opening. |
背景资料 | Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure.For these reasons,it is often extremely difficult to generate antibodies with specificities to collagens.The development of &type* specific antibodies is dependent on NON-DENATURED three-dimensional epitopes.Biorab extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation.This preparation results in a native conformation of the protein.Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies.Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. |
Uniprot ID | P12109/P12110/P12111 |
推荐稀释比 | ELISA:1:5,000 - 1:20,000 IHC:1:50 - 1:200 IP:1:100 WB:1:500 - 1:5,000 |
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